P-145: Efficacy of Simvasiatin in The Prevention of Doxorubicin-Induced Testicular Morphometric-Stereological Alterations in Mice

Background: Doxorubicin (DOX), a widely used antineoplastic compound, is associated with testicular damage and infertility. In this study, we intended to determine the possible preventive effects of simvastatin (SIM), a lipid lowering agent with antioxidant and anti-inflammatory activities, on testicular morphometric-stereological changes due to DOX treatment in mice. Materials and Methods: Male mice, five weeks old, were randomly divided into four groups of six mice each. DOX was administered to two groups of mice in 5 equal intraperitoneal injections over a period of 4 weeks (accumulated dose of 20 mg/kg). One of these groups received 5 equal oral doses of SIM (accumulated dose of 60 mg/kg) along with DOX. A vehicle-treated control group and a SIM control group were also included. Morphometric measurement was determined by using calibrated ocular micrometer.Results: Histomorphometrical examinations revealed significant reductions in the seminiferous tubules diameters and their epithelial heights along with an increase in testicular capsule thickness and interstitial space widening in DOX-treated mice. All above-mentioned parameters were recovered significantly by SIM co-administration. Conclusion: These results have indicated that SIM may have a preventive role on morphometric-stereological changes caused by DOX in mouse testes.